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Chapters
- 00:05Title
- 00:41Preparation of Tagged Chromatin
- 04:16Chromatin Immunoprecipitation
- 06:41Immunoprecipitated Chromatin Analysis
- 08:08Results: enChIP to Telomere and the Interferon Regulatory Factor-1 (IRF-1) Promoter Regions
- 09:00Conclusion
The identification of molecules associated with specific genomic regions of interest is required to understand the mechanisms of regulation of the functions of these regions. This protocol describes procedures to perform engineered DNA-binding molecule-mediated chromatin imunoprecipitation (enChIP) for identification of proteins and RNAs associated with a specific genomic region.
