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黄曲霉毒素在花生RNAi介导的控制：方法分析霉菌毒素的产生和转基因表达的花生/<em>黑</em>病害系统
JoVE 杂志
环境科学
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JoVE 杂志 环境科学
RNAi-mediated Control of Aflatoxins in Peanut: Method to Analyze Mycotoxin Production and Transgene Expression in the Peanut/Aspergillus Pathosystem
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黄曲霉毒素在花生RNAi介导的控制：方法分析霉菌毒素的产生和转基因表达的花生/<em>黑</em>病害系统

20,589 Views

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09:44 min

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December 21, 2015

DOI:

10.3791/53398-v

DOI:
10.3791/53398-v

•

09:44 min
December 21, 2015

•

14 Views
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1National Peanut Research Laboratory,United States Department of Agriculture, Agricultural Research Service

Summary

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我们证明了包含RNA干扰信号沉默中黄曲霉毒素合成基因真菌黄曲霉黄曲霉毒素的转基因表达的花生种子的分析方法。真菌毒素的植物中RNAi介导的控制以前没有被报道。

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RNAiAflatoxinsPeanutAspergillusMycotoxinReal-time PCRUPLCTransgenicFood ContaminationFood Safety

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Arias, R. S., Dang, P. M., Sobolev, V. S. RNAi-mediated Control of Aflatoxins in Peanut: Method to Analyze Mycotoxin Production and Transgene Expression in the Peanut/Aspergillus Pathosystem. J. Vis. Exp. (106), e53398, doi:10.3791/53398 (2015).

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