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La comparación de la afinidad de unión a proteínas GTPasa-utilizando ensayos de competición
JoVE 杂志
生物学
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JoVE 杂志 生物学
Comparing the Affinity of GTPase-binding Proteins using Competition Assays
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La comparación de la afinidad de unión a proteínas GTPasa-utilizando ensayos de competición

8,858 Views

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10:37 min

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October 08, 2015

DOI:

10.3791/53254-v

DOI:
10.3791/53254-v

•

10:37 min
October 08, 2015

•

1 Views
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1School of Biochemistry,University of Bristol, 2Department of Biomedical Science,University of Sheffield

Tags

GTPase-binding ProteinsCompetition AssaysRac1GDPGTPGFP TagPurified ProteinsBinding CapabilitiesProtein InteractionsImmunoprecipitationSignalingMammalian Cells

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Cite this Article

Williamson, R. C., Bass, M. D. Comparing the Affinity of GTPase-binding Proteins using Competition Assays. J. Vis. Exp. (104), e53254, doi:10.3791/53254 (2015).

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