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Het vergelijken van de affiniteit van GTPase-bindende eiwitten met behulp competitiebepalingen

JoVE 杂志
生物学

This content is Free Access.

JoVE 杂志生物学

Comparing the Affinity of GTPase-binding Proteins using Competition Assays

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Het vergelijken van de affiniteit van GTPase-bindende eiwitten met behulp competitiebepalingen

8,858 Views

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10:37 min

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October 08, 2015

DOI:

10.3791/53254-v

DOI:

10.3791/53254-v

•

10:37 min

October 08, 2015

•

1 Views

Rosalind C. Williamson, Mark D. Bass

¹School of Biochemistry,University of Bristol, ²Department of Biomedical Science,University of Sheffield

Tags

GTPase-binding Proteins Competition Assays Rac1 GDP GTP GFP Tag Purified Proteins Binding Capabilities Protein Interactions Immunoprecipitation Signaling Mammalian Cells

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