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셀 성인 마우스 뇌실 영역에서 신경 줄기 및 전구 세포의 정렬과 세포주기 역학의 라이브 이미징
JoVE 杂志
神经科学
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JoVE 杂志 神经科学
Cell Sorting of Neural Stem and Progenitor Cells from the Adult Mouse Subventricular Zone and Live-imaging of their Cell Cycle Dynamics
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셀 성인 마우스 뇌실 영역에서 신경 줄기 및 전구 세포의 정렬과 세포주기 역학의 라이브 이미징

13,874 Views

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09:27 min

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September 14, 2015

DOI:

10.3791/53247-v

DOI:
10.3791/53247-v

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09:27 min
September 14, 2015

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1 Views
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1CEA DSV iRCM SCSR, Laboratoire de Radiopathologie, UMR 967, 2INSERM, UMR 967, 3Université Paris Diderot, Sorbonne Paris Cité, UMR 967, 4Université Paris Sud, UMR 967, 5CNRS, Université Paris Sud, UMR 9197, Neuroscience Paris-Saclay Institute, Molecules Circuits Department

Summary

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We report a Fluorescent Activated Cell Sorting (FACS)-based method to isolate neural stem cells (NSCs) and their progeny from the subventricular zone (SVZ) of the adult mouse brain. Applied to Fluorescence Ubiquitination Cell Cycle Indicator (FUCCI) transgenic mice, it allows the study of cell cycle progression by live imaging.

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Cite this Article

Daynac, M., Morizur, L., Kortulewski, T., Gauthier, L. R., Ruat, M., Mouthon, M., Boussin, F. D. Cell Sorting of Neural Stem and Progenitor Cells from the Adult Mouse Subventricular Zone and Live-imaging of their Cell Cycle Dynamics. J. Vis. Exp. (103), e53247, doi:10.3791/53247 (2015).

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