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Besleyici içermeyen şartlar Sendai virüs kullanılarak insan periferik T hücrelerinden uyarılmış pluripotent kök hücrelerin üretilmesi
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发育生物学
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JoVE 杂志 发育生物学
Generation of Induced Pluripotent Stem Cells from Human Peripheral T Cells Using Sendai Virus in Feeder-free Conditions
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Besleyici içermeyen şartlar Sendai virüs kullanılarak insan periferik T hücrelerinden uyarılmış pluripotent kök hücrelerin üretilmesi

11,734 Views

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09:32 min

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November 11, 2015

DOI:

10.3791/53225-v

DOI:
10.3791/53225-v

•

09:32 min
November 11, 2015

•

9 Views
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1Department of Cardiology,Keio University School of Medicine, 2Department of Emergency and Critical Care Medicine,Keio University School of Medicine

Summary

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This protocol describes how to generate induced pluripotent stem cells (iPSCs) from human peripheral T cells in feeder-free conditions using a combination of matrigel and Sendai virus vectors containing reprogramming factors.

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Induced Pluripotent Stem CellsIPSCsPeripheral T CellsSendai VirusFeeder-free ConditionsRegenerative TherapiesT Cell ReceptorAntigen-specific T CellsGenome RearrangementClinical ApplicationFetal Bovine SerumFeeder CellsBiosafetyActivated T Cells

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Cite this Article

Kishino, Y., Seki, T., Yuasa, S., Fujita, J., Fukuda, K. Generation of Induced Pluripotent Stem Cells from Human Peripheral T Cells Using Sendai Virus in Feeder-free Conditions. J. Vis. Exp. (105), e53225, doi:10.3791/53225 (2015).

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