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Chapters
- 00:05Title
- 01:41Preparation of Y. enterocolitica Strains for Cell Culture Infection
- 03:38Infection of HeLa Cells and Loading with CCF4
- 05:44Laser Scanning Microscopy
- 07:17Image Quantification
- 10:52Results: Analysis of Yersinia enterocolitica Effector Translocation
- 11:54Conclusion
Effector translocation into host cells via a type III secretion system is a common virulence strategy among gram-negative bacteria. A beta-lactamase effector fusion based assay for quantitative analysis of translocation was applied. In Yersinia infected cells, conversion of a FRET reporter by the beta-lactamase is monitored using laser scanning microscopy.
