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Chapters
- 00:05Title
- 01:13Fibroblast-collagen Matrix Continuous Culture
- 03:35Immune and Lung Epithelial Cell Seeding
- 05:423D Lung Tissue Model Air Exposure, Harvest and Mounting
- 07:123D Model Visualization, Acquisition and Quantitative Analysis
- 08:26Results: Representative 3D Visualization and Quantitative Tissue Model Analysis
- 09:39Conclusion
Human tuberculosis infection is a complex process, which is difficult to model in vitro. Here we describe a novel 3D human lung tissue model that recapitulates the dynamics that occur during infection, including the migration of immune cells and early granuloma formation in a physiological environment.
