Ultrasonic vocalizations (USVs) in mice differ depending on age, sex, condition, and genetic background. Using two ultrasound emitters broadcasting simultaneously in different locations, this two-choice test can evaluate murine recognition and preference responses to different characteristics of USVs.
Asaba, A., Kato, M., Koshida, N., Kikusui, T. Determining Ultrasonic Vocalization Preferences in Mice using a Two-choice Playback Test. J. Vis. Exp. (103), e53074, doi:10.3791/53074 (2015).