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Enriquecimento de Proteínas da Matriz Extracelular a partir de tecidos e digestão em péptidos para Análise de Espectrometria de Massa
JoVE 杂志
生物学
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JoVE 杂志 生物学
Enrichment of Extracellular Matrix Proteins from Tissues and Digestion into Peptides for Mass Spectrometry Analysis

Enriquecimento de Proteínas da Matriz Extracelular a partir de tecidos e digestão em péptidos para Análise de Espectrometria de Massa

25,941 Views

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07:28 min

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July 23, 2015

DOI:

10.3791/53057-v

DOI:
10.3791/53057-v

•

07:28 min
July 23, 2015

•

31 Views
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1Koch Institute for Integrative Cancer Research,Massachusetts Institute of Technology, 2Proteomics Platform,Broad Institute

Summary

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This protocol describes a procedure for enriching ECM proteins from tissues or tumors and deglycosylating and digesting the ECM-enriched preparations into peptides to analyze their protein composition by mass spectrometry.

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Extracellular MatrixECMDigestionPeptidesMass SpectrometryDecellularizationEnrichmentCross-linked ProteinsGlycosylationBiomarkersDiagnosticTherapeutic Targets

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Cite this Article

Naba, A., Clauser, K. R., Hynes, R. O. Enrichment of Extracellular Matrix Proteins from Tissues and Digestion into Peptides for Mass Spectrometry Analysis. J. Vis. Exp. (101), e53057, doi:10.3791/53057 (2015).

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