Journal
/
生物学
/
从富集组织及消化细胞外基质蛋白的成肽质谱分析
JoVE 杂志
生物学
This content is Free Access.
JoVE 杂志 生物学
Enrichment of Extracellular Matrix Proteins from Tissues and Digestion into Peptides for Mass Spectrometry Analysis
Please note that all translations are automatically generated. Click here for the English version.

从富集组织及消化细胞外基质蛋白的成肽质谱分析

25,941 Views

•

07:28 min

•

July 23, 2015

DOI:

10.3791/53057-v

DOI:
10.3791/53057-v

•

07:28 min
July 23, 2015

•

31 Views
, ,
1Koch Institute for Integrative Cancer Research,Massachusetts Institute of Technology, 2Proteomics Platform,Broad Institute

Summary

Automatically generated

This protocol describes a procedure for enriching ECM proteins from tissues or tumors and deglycosylating and digesting the ECM-enriched preparations into peptides to analyze their protein composition by mass spectrometry.

Explore More Videos

Extracellular MatrixECMDigestionPeptidesMass SpectrometryDecellularizationEnrichmentCross-linked ProteinsGlycosylationBiomarkersDiagnosticTherapeutic Targets

Read Article

Cite this Article

Naba, A., Clauser, K. R., Hynes, R. O. Enrichment of Extracellular Matrix Proteins from Tissues and Digestion into Peptides for Mass Spectrometry Analysis. J. Vis. Exp. (101), e53057, doi:10.3791/53057 (2015).

Download .ris file

Copy

Share Video

.

Copy