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Chapters
- 00:05Title
- 01:58Culturing the Cerebellar Granule Cells
- 04:47Selectively Depleting the Microglia at 6 Days In Vitro
- 06:35Results: Characterization of LME Treatment on CGCs and Neurons and Astrocytes in CGC Cultures
- 07:51Conclusion
Microglia can influence neurons and other glia in culture by various non-cell autonomous mechanisms. Here, we present a protocol to selectively deplete microglia from primary neuronal cultures. This method has the potential to elucidate the role of microglial-neuronal interactions, with implications for neurodegenerative conditions where neuroinflammation is a hallmark feature.
