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Herstellung von Mica und Siliziumsubstraten für die DNA-Origami-Analyse und Experimentieren
JoVE 杂志
化学
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JoVE 杂志 化学
Preparation of Mica and Silicon Substrates for DNA Origami Analysis and Experimentation
DOI:

12:03 min

July 23, 2015

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Chapters

  • 00:05Title
  • 02:11Depositing DNA Origami on Mica
  • 03:35Complimentary Metal-oxide Semiconductor (CMOS) / Silicon Cleaning Set-up
  • 05:56Preparing and Cleaning the Silicon Substrate
  • 08:58Depositing DNA Origami on APTES-functionalized Silicon
  • 10:18Results: Changes in Sample Coverage with Substrate Type and Solution Concentration
  • 11:16Conclusion

Summary

自动翻译

Reproducible cleaning processes for substrates used in DNA origami research are described, including bench-top RCA cleaning and derivatization of silicon oxide. Protocols for surface preparation, DNA origami deposition, drying parameters, and simple experimental set-ups are illustrated.

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