Journal
/
/
Administración intranasal de vacunas contra la influenza recombinante en modelos de ratón quimérico para el Estudio de la mucosa Inmunidad
JoVE 杂志
免疫与感染
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 免疫与感染
Intranasal Administration of Recombinant Influenza Vaccines in Chimeric Mouse Models to Study Mucosal Immunity
DOI:

10:39 min

June 25, 2015

, , ,

Chapters

  • 00:05Title
  • 01:49Generation of Recombinant Live Attentuated Influenza Vaccines by Reverse Genetics
  • 04:03Inoculating Eggs and Harvesting Allantoic Fluid
  • 05:02Tracking Vaccine-Specific Immunity: Peptide-Bearing Dendritic Cells
  • 06:06Tracking Vaccine-Specific Immunity: Vaccine-Specific T Cell Proliferation
  • 07:05Mouse Irradiation, Transplantation, Vaccination and Targeted Depletion
  • 08:24Results: Modulation of Mucosal Immunity by Live Attentuated Virus; Role of CD103+ Dendritic Cells in Vaccine Induced Immunity
  • 10:01Conclusion

Summary

自动翻译

There is an overall lack of knowledge about how vaccines work. Here we propose the combined use of reverse genetics and bone marrow chimeric mice to gain insight into the early host immune responses to vaccines with a special focus on dendritic cells and T cell immunity.

Related Videos

Read Article