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隔离和卫星细胞的表征鼠头Branchiomeric肌肉
JoVE 杂志
发育生物学
This content is Free Access.
JoVE 杂志 发育生物学
Isolation and Characterization of Satellite Cells from Rat Head Branchiomeric Muscles
DOI:

07:37 min

July 20, 2015

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Chapters

  • 00:05Title
  • 01:33Head Muscle (Masseter, Digastric, and Levator Veli Palatini) Dissection
  • 04:01Satellite Cell Isolation and Culture
  • 06:18Results: Representative Myotube Generation from Rat Head Branchiometric Muscle Tissue
  • 07:09Conclusion

Summary

自动翻译

这个协议描述了从一个9周龄大鼠branchiomeric头肌卫星细胞的分离。肌肉从不同的鳃弓起源。随后,卫星细胞在局部上光毫米大小来研究其分化培养。这种方法避免了卫星细胞的扩增和传代。

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