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Mus Naïve CD4<sup> +</sup> T Cell Isolation og<em> In vitro</em> Differentiering til T-celledelmængder
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Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets

Mus Naïve CD4<sup> +</sup> T Cell Isolation og<em> In vitro</em> Differentiering til T-celledelmængder

DOI:
10.3791/52739-v

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07:12 min

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April 16, 2015

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,
1Department of Microbiology and Immunology, The Chicago Medical School,Rosalind Franklin University of Medicine and Science

Chapters

  • 00:05Title
  • 01:09Mouse Lymph Node and Spleen Isolation
  • 02:11Tissue Processing and CD4+ Enrichment
  • 04:22Naïve CD4+ T Cell Sorting and In vitro Differentiation
  • 05:49Results: Representative In vitro Th Differentiation Data
  • 06:40Conclusion

Summary

自动翻译

自动翻译

Naïve CD4+ T cells polarize to various subsets depending on the environment at the time of activation. The differentiation of naïve CD4+ T cells to various effector subsets can be achieved in vitro through the addition of T cell receptor stimuli and specific cytokine signals.

Tags

Keywords: MouseNaive CD4+ T CellsT Cell Receptor (TCR)MHC Class IICytokineT Helper Cell DifferentiationT Cell SubsetsIn Vitro DifferentiationEffector T Cells

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