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免疫组化可视化小鼠肠神经系统的发展
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发育生物学
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JoVE 杂志 发育生物学
Immunostaining to Visualize Murine Enteric Nervous System Development
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免疫组化可视化小鼠肠神经系统的发展

11,152 Views

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07:54 min

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April 29, 2015

DOI:

10.3791/52716-v

DOI:
10.3791/52716-v

•

07:54 min
April 29, 2015

•

9 Views
, , ,
1Department of Surgery,University of Wisconsin School of Medicine and Public Health, 2Department of Neurosciences,University of Wisconsin School of Medicine and Public Health, 3Department of Pediatrics,University of Wisconsin School of Medicine and Public Health

Summary

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The enteric nervous system is formed by neural crest cells that proliferate, migrate and colonize the gut. Neural crest cells differentiate into neurons with markers specific for their neurotransmitter phenotype. This protocol describes a technique for dissecting, fixing and immunostaining of the murine embryonic gastrointestinal tract to visualize enteric nervous system neurotransmitter expression.

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Enteric Nervous SystemNeural Crest CellsCholinergic NeuronsCholine Acetyltransferase (ChAT)ImmunostainingEmbryonic DevelopmentGastrointestinal TractNeurotransmitter Expression

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Cite this Article

Barlow-Anacker, A. J., Erickson, C. S., Epstein, M. L., Gosain, A. Immunostaining to Visualize Murine Enteric Nervous System Development. J. Vis. Exp. (98), e52716, doi:10.3791/52716 (2015).

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