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Biolistic Transformation of a Fluorescent Tagged Gene into the Opportunistic Fungal Pathogen Cryptococcus neoformans
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Biolistic Transformation of a Fluorescent Tagged Gene into the Opportunistic Fungal Pathogen Cryptococcus neoformans

Biolistic Transformation of a Fluorescent Tagged Gene into the Opportunistic Fungal Pathogen Cryptococcus neoformans

9,776 Views

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07:32 min

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March 19, 2015

DOI:

10.3791/52666-v

DOI:
10.3791/52666-v

•

07:32 min
March 19, 2015

•

2 Views
, , ,
1Department of Genetics and Biochemistry, Eukaryotic Pathogens Innovation Center (EPIC),Clemson University, 2Department of Biology,Western Carolina University

Summary

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Biolistic transformation is a method used to generate stable integration of DNA into the genome of the opportunistic pathogen Cryptococcus neoformans through homologous recombination. We will demonstrate biolistic transformation of a construct, which has the gene encoding acetate kinase fused to the fluorescent tag mCherry into C. neoformans.

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Taylor, T., Bose, I., Luckie, T., Smith, K. Biolistic Transformation of a Fluorescent Tagged Gene into the Opportunistic Fungal Pathogen Cryptococcus neoformans. J. Vis. Exp. (97), e52666, doi:10.3791/52666 (2015).

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