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发育生物学
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Derivation of Highly Purified Cardiomyocytes from Human Induced Pluripotent Stem Cells Using Small Molecule-modulated Differentiation and Subsequent Glucose Starvation
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JoVE 杂志 发育生物学
Derivation of Highly Purified Cardiomyocytes from Human Induced Pluripotent Stem Cells Using Small Molecule-modulated Differentiation and Subsequent Glucose Starvation

Derivation of Highly Purified Cardiomyocytes from Human Induced Pluripotent Stem Cells Using Small Molecule-modulated Differentiation and Subsequent Glucose Starvation

DOI:
10.3791/52628-v

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11:53 min

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March 18, 2015

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, , , , ,
1Stanford Cardiovascular Institute,Stanford University School of Medicine, 2Institute of Stem Cell Biology and Regenerative Medicine, Cardiovascular Medicine Division, Department of Medicine, Child Health Research Institute,Stanford University School of Medicine

Chapters

  • 00:05Title
  • 01:56Passaging of hiPSCs
  • 04:52Cardiac Differentiation of Human iPSC
  • 07:17Purification of Human Cardiomyocytes through Glucose Starvation
  • 09:39Representative Analysis of Differentiated Cardiomyocytes
  • 10:55Conclusion

Summary

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Here, we describe a robust protocol for human cardiomyocyte derivation that combines small molecule-modulated cardiac differentiation and glucose deprivation-mediated cardiomyocyte purification, enabling production of purified cardiomyocytes for the purposes of cardiovascular disease modeling and drug screening.

Tags

Human Induced Pluripotent Stem CellsCardiomyocyte DifferentiationSmall Molecule-based ProtocolWnt/β-Catenin SignalingGlucose StarvationPurified CardiomyocytesIn Vitro Disease ModelingDrug Screening

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