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전체 양자 간 길 탈 신경과 결합 시간 차등 염색 기술은 물고기에 Ionocytes을 연구하기 위해
JoVE 杂志
发育生物学
This content is Free Access.
JoVE 杂志 发育生物学
A Time Differential Staining Technique Coupled with Full Bilateral Gill Denervation to Study Ionocytes in Fish
DOI:

11:24 min

March 19, 2015

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Chapters

  • 00:05Title
  • 01:30Time Differential Staining Technique: Mitochondria-specific Dye Bath
  • 02:44Full Bilateral Denervation Procedure
  • 05:31Time Differential Staining Technique: Immunohistochemistry
  • 08:26Mounting and Imaging
  • 09:37Results: Distribution and Innervation of Ionocytes on a Single Filament of the First Gill Arch of Goldfish Acclimated to 25 oC
  • 10:55Conclusion

Summary

自动翻译

This manuscript describes a protocol to track the re-distribution of branchial ionocytes and their innervation using a time differential staining technique coupled with full bilateral gill denervation.

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