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Super-résolution de l'imagerie cellulaire Synapse immunologique Natural Killer sur un verre soutenu par Planar lipidique en deux couches
JoVE 杂志
免疫与感染
This content is Free Access.
JoVE 杂志 免疫与感染
Super-resolution Imaging of the Natural Killer Cell Immunological Synapse on a Glass-supported Planar Lipid Bilayer
DOI:

09:56 min

February 11, 2015

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Chapters

  • 00:05Title
  • 01:52Assembling the Glass-supported Planar Lipid Bilayer
  • 06:17Imaging of the NK Synapse on Lipid Bilayer Using Stimulated Emission Depletion (STED)
  • 08:11Results: Super-resolution Images of NK Cell Synapse on the Lipid Bilayer Using STED Microscope
  • 09:21Conclusion

Summary

自动翻译

We describe here a combination of the glass-supported lipid bilayer technique of forming immunological synapses with the super-resolution imaging technique of stimulated emission depletion (STED) microscopy. The goal of this protocol is to provide users with the instructions necessary to successfully carry out these two techniques.

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