Journal
/
/
भ्रूण कृंतक दिमाग से प्राथमिक Mesencephalic डोपामिनर्जिक न्यूरॉन्स के अलगाव, संस्कृति और लंबी अवधि के रखरखाव
JoVE 杂志
神经科学
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 神经科学
Isolation, Culture and Long-Term Maintenance of Primary Mesencephalic Dopaminergic Neurons From Embryonic Rodent Brains
DOI:

08:45 min

February 19, 2015

, , ,

Chapters

  • 00:05Title
  • 01:29Making Preparations
  • 02:45Dissecting the Embryonic Ventral Midbrain
  • 04:44Dissociating the Ventral Midbrain Cells
  • 06:09Plating and Culturing the Ventral Midbrain Cells
  • 07:49Results: Characterizing the Dopaminergic Neuron Cultures
  • 08:21Conclusion

Summary

自动翻译

The causes of degeneration of midbrain dopaminergic neurons during Parkinson’s disease are not fully understood. Cellular culture systems provide an essential tool for study of the neurophysiological properties of these neurons. Here we present an optimized protocol, which can be utilized for in vitro modeling of neurodegeneration.

Related Videos

Read Article