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Validatie van Nanobody en antilichamen gebaseerde<em> In Vivo</em> Tumor Xenograft NIRF-imaging Experimenten op muizen, waarbij<em> Ex Vivo</em> Flowcytometrie en Microscopie
JoVE 杂志
医学
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JoVE 杂志 医学
Validation of Nanobody and Antibody Based In Vivo Tumor Xenograft NIRF-imaging Experiments in Mice Using Ex Vivo Flow Cytometry and Microscopy
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Validatie van Nanobody en antilichamen gebaseerde<em> In Vivo</em> Tumor Xenograft NIRF-imaging Experimenten op muizen, waarbij<em> Ex Vivo</em> Flowcytometrie en Microscopie

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08:09 min

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April 06, 2015

DOI:

10.3791/52462-v

DOI:
10.3791/52462-v

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08:09 min
April 06, 2015

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1Department of Diagnostic and Interventional Radiology,University Medical Center, Hamburg, 2Institute of Immunology,University Medical Center, Hamburg, 3University Cancer Center Hamburg,University Medical Center, Hamburg, 4Department of Oncology and Hematology,University Medical Center, Hamburg

Summary

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This protocol outlines the steps required to perform ex vivo validation of in vivo near-infrared fluorescence xenograft imaging experiments in mice using fluorophore labelled nanobodies and conventional antibodies.

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NanobodyAntibodyIn VivoTumor XenograftNIRF ImagingFlow CytometryMicroscopySubcutaneous TumorAntigen-negativeAntigen-positiveAlexaFluor680Non-invasive ImagingEx Vivo Validation

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Bannas, P., Lenz, A., Kunick, V., Fumey, W., Rissiek, B., Schmid, J., Haag, F., Leingärtner, A., Trepel, M., Adam, G., Koch-Nolte, F. Validation of Nanobody and Antibody Based In Vivo Tumor Xenograft NIRF-imaging Experiments in Mice Using Ex Vivo Flow Cytometry and Microscopy. J. Vis. Exp. (98), e52462, doi:10.3791/52462 (2015).

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