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Pluripotente menselijke stamcellen Gebaseerd Ontwikkelingstoxiciteit Testen voor Chemical Safety Screening and Systems Biology gegevens Generation
1Center of Physiology and Pathophysiology, Institute of Neurophysiology,University of Cologne, 2Department of Biology,University of Konstanz, 3Department of Statistics,Technical University of Dortmund, 4Leibniz Research Centre for Working Environment and Human Factors,Technical University of Dortmund
Chapters
- 00:05Title
- 02:02H9 Cell Culture on Basement Membrane Matrix Coated Plates
- 04:40Embryoid Bodies Formation
- 06:17Collection of Embryoid Bodies and Treatment
- 07:55RNA Isolation
- 09:31Splitting and Maintenance of H9 Cells
- 10:55Differentiation of hESC towards Neuroectodermal Progenitor Cells (NEP)
- 15:06Results: Methyl Mercury Exposure Causes Gene Expression Changes in Embryoid Bodies
- 16:40Conclusion
De protocollen beschrijven twee in vitro ontwikkelingstoxiciteit testsystemen (UKK en UKN1) op basis van menselijke embryonale stamcellen en transcriptoom studies. De testsystemen voorspellen mens ontwikkelingsstoornissen giftigheidsgevaar, en kunnen bijdragen aan dierproeven, de kosten en de tijd die nodig is voor de chemische veiligheid testen te verminderen.
Tags
Human Pluripotent Stem CellsDevelopmental Toxicity AssaysChemical Safety ScreeningSystems BiologyEmbryonic Stem CellsEmbryoid BodiesNeuroectodermal Progenitor CellsTranscriptome MicroarrayIn Vitro Toxicity TestingEarly Embryonic ToxicityTeratogenicityDevelopmental NeurotoxicityEpigenetic ChangesToxicity BiomarkersSystems Biology AnalysisAnimal Studies ReductionDrug DevelopmentChemical Safety Evaluation
