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Hele Mount Labeling van Cilia in het Main Olfactorische Systeem van Muizen
JoVE 杂志
神经科学
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JoVE 杂志 神经科学
Whole Mount Labeling of Cilia in the Main Olfactory System of Mice

Hele Mount Labeling van Cilia in het Main Olfactorische Systeem van Muizen

DOI:

08:42 min

December 27, 2014

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Chapters

  • 00:05Title
  • 01:24Preparing the Nasal Septum
  • 03:12Isolating the Olfactory Epithelium
  • 05:01Staininig the Epithelium
  • 06:53Results: Images of Olfactory Epithelium
  • 08:04Conclusion

Summary

自动翻译

Cilia of olfactory sensory neurons contain proteins of the signal transduction cascade, but a detailed spatial analysis of their distribution is difficult in cryosections. We describe here an optimized approach for whole mount labeling and en face visualization of ciliary proteins.

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