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Imagem Intravital de axonais Interações com Microglia e macrófagos em um mouse Dorsal Coluna lesão por esmagamento
JoVE 杂志
神经科学
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JoVE 杂志 神经科学
Intravital Imaging of Axonal Interactions with Microglia and Macrophages in a Mouse Dorsal Column Crush Injury

Imagem Intravital de axonais Interações com Microglia e macrófagos em um mouse Dorsal Coluna lesão por esmagamento

11,177 Views

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08:43 min

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November 23, 2014

DOI:

10.3791/52228-v

DOI:
10.3791/52228-v

•

08:43 min
November 23, 2014

•

3 Views
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1Department of Neurosciences,Case Western Reserve University, 2Department of Biomedical Engineering,Case Western Reserve University, 3Department of Pediatrics,Case Western Reserve University

Summary

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Two-photon intravital imaging can be used to investigate interactions among different cell types in the spinal cord in their native tissue environment in a bone marrow chimeric animal with a dorsal column traumatic spinal cord crush injury.

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Intravital ImagingAxonal InteractionsMicrogliaMacrophagesMouseDorsal Column Crush InjurySpinal Cord InjuryTwo-photon MicroscopyBone Marrow ChimeraLaminectomy

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Cite this Article

Evans, T. A., Barkauskas, D. S., Myers, J. T., Huang, A. Y. Intravital Imaging of Axonal Interactions with Microglia and Macrophages in a Mouse Dorsal Column Crush Injury. J. Vis. Exp. (93), e52228, doi:10.3791/52228 (2014).

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