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High Throughput Fluorometric Technik zur Bewertung der Makrophagen-Phagozytose und Aktinpolymerisation
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JoVE 杂志 免疫与感染
High Throughput Fluorometric Technique for Assessment of Macrophage Phagocytosis and Actin Polymerization
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High Throughput Fluorometric Technik zur Bewertung der Makrophagen-Phagozytose und Aktinpolymerisation

14,380 Views

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09:22 min

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November 27, 2014

DOI:

10.3791/52195-v

DOI:
10.3791/52195-v

•

09:22 min
November 27, 2014

•

2 Views
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1Department of Pharmacology,University of Minnesota, 2Department of Surgery,University of Minnesota, 33M Corporate Research Laboratory

Summary

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Here we present a protocol to quantify phagocytosis of fluorescent particles by adherent macrophage cell line using a fluorometric method. This method facilitates a high throughput quantification of particle internalization as well as the resulting actin polymerization.

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Cite this Article

Ninković, J., Roy, S. High Throughput Fluorometric Technique for Assessment of Macrophage Phagocytosis and Actin Polymerization. J. Vis. Exp. (93), e52195, doi:10.3791/52195 (2014).

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