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Viral Determinazione Concentrazione Attraverso placca saggi: Sistemi overlay per mezzo di tradizionali e nuovi
JoVE 杂志
免疫与感染
This content is Free Access.
JoVE 杂志 免疫与感染
Viral Concentration Determination Through Plaque Assays: Using Traditional and Novel Overlay Systems

Viral Determinazione Concentrazione Attraverso placca saggi: Sistemi overlay per mezzo di tradizionali e nuovi

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09:28 min

November 04, 2014

DOI:

09:28 min
November 04, 2014

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Summary

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Plaque assays are the gold standard for viral quantification, utilizing entrapping overlays on host cellular monolayers to determine viral titers. While various semisolid overlays have traditionally been used, here we demonstrate plaque techniques comparing semisolid overlays to a novel liquid microcrystalline cellulose among several families of viruses.

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