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ヒト骨格筋からの一次筋原細胞および線維芽細胞の単離および定量的免疫組織化学的特性評価
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Isolation and Quantitative Immunocytochemical Characterization of Primary Myogenic Cells and Fibroblasts from Human Skeletal Muscle
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ヒト骨格筋からの一次筋原細胞および線維芽細胞の単離および定量的免疫組織化学的特性評価

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11:22 min

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January 12, 2015

DOI:

10.3791/52049-v

DOI:
10.3791/52049-v

•

11:22 min
January 12, 2015

•

4 Views
, , , ,
1Centre of Human and Aerospace Physiological Sciences,King’s College London, 2Wellcome Trust-Medical Research Council,Cambridge Stem Cell Institute

Tags

Skeletal MuscleSatellite CellsMyogenic CellsMuscle Precursor CellsFibroblastsCD56DesminTE-7Enzymatic DigestionCollagenaseDispaseMagnetic Activated Cell Sorting (MACS)ImmunocytochemistryCell CultureMuscle Regeneration

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Agley, C. C., Rowlerson, A. M., Velloso, C. P., Lazarus, N. L., Harridge, S. D. R. Isolation and Quantitative Immunocytochemical Characterization of Primary Myogenic Cells and Fibroblasts from Human Skeletal Muscle. J. Vis. Exp. (95), e52049, doi:10.3791/52049 (2015).

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