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בידוד וכמוני Immunocytochemical אפיון של תאים ראשוניים myogenic וFibroblasts משרירי שלד אדם
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发育生物学
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Isolation and Quantitative Immunocytochemical Characterization of Primary Myogenic Cells and Fibroblasts from Human Skeletal Muscle
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בידוד וכמוני Immunocytochemical אפיון של תאים ראשוניים myogenic וFibroblasts משרירי שלד אדם

15,618 Views

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11:22 min

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January 12, 2015

DOI:

10.3791/52049-v

DOI:
10.3791/52049-v

•

11:22 min
January 12, 2015

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4 Views
, , , ,
1Centre of Human and Aerospace Physiological Sciences,King’s College London, 2Wellcome Trust-Medical Research Council,Cambridge Stem Cell Institute

Tags

Skeletal MuscleSatellite CellsMyogenic CellsMuscle Precursor CellsFibroblastsCD56DesminTE-7Enzymatic DigestionCollagenaseDispaseMagnetic Activated Cell Sorting (MACS)ImmunocytochemistryCell CultureMuscle Regeneration

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Cite this Article

Agley, C. C., Rowlerson, A. M., Velloso, C. P., Lazarus, N. L., Harridge, S. D. R. Isolation and Quantitative Immunocytochemical Characterization of Primary Myogenic Cells and Fibroblasts from Human Skeletal Muscle. J. Vis. Exp. (95), e52049, doi:10.3791/52049 (2015).

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