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Chapters
- 00:05Title
- 01:20Organotypic Entorhino-hippocampal Slice Cultures
- 02:37In Vitro Hypoxia and Oxygen-glucose Deprivation (OGD) with Reperfusion
- 04:28Fixation and Immunostaining
- 05:47Determination of Regional Vessel Density
- 06:22Results: Neuronal Death and Blood Vessel Loss after Hypoxia or OGD
- 07:41Conclusion
A protocol for entorhino-hippocampal organotypic slice cultures, which allows reproducing many aspects of ischemic brain injury, is presented. By studying changes of the neurovasculature in addition to changes in the neurons, this protocol is a versatile tool to study plastic changes in neural tissue after injury.
