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Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope
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August 15, 2014
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A module for single plane illumination microscopy (SPIM) is described which is easily adapted to an inverted wide-field microscope and optimized for 3-dimensional cell cultures. The sample is located within a rectangular capillary, and via a microfluidic system fluorescent dyes, pharmaceutical agents or drugs can be applied in small quantities.
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Bruns, T., Schickinger, S., Schneckenburger, H. Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope. J. Vis. Exp. (90), e51993, doi:10.3791/51993 (2014).
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