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神经科学
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Isolation and Culture of Dissociated Sensory Neurons From Chick Embryos
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神经科学
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JoVE 杂志 神经科学
Isolation and Culture of Dissociated Sensory Neurons From Chick Embryos

Isolation and Culture of Dissociated Sensory Neurons From Chick Embryos

DOI:
10.3791/51991-v

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11:16 min

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September 24, 2014

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, ,
1Department of Natural Sciences,Assumption College

Chapters

  • 00:05Title
  • 01:48Chick Dissection and Isolation of DRGs
  • 04:54Dissociation, Enriching, and Culturing DRG Neurons – Part 1
  • 07:29Dissociation, Enriching, and Culturing DRG Neurons – Part 2
  • 09:09Results: Isolation and Culture of Dissociated Sensory Neurons from Chick Embryos
  • 10:12Conclusion

Summary

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自动翻译

Cell culture models provide detailed control over environmental conditions and thus provide a powerful platform to elucidate numerous aspects of neuronal cell biology. We describe a rapid, inexpensive, and reliable method to isolate, dissociate, and culture sensory neurons from chick embryos. Details of substrata preparation and immunocytochemistry are also provided.

Tags

Neuron IsolationCell CultureChick EmbryoSensory NeuronsPrimary NeuronsLaminin SubstrateIn Vitro ModelImmunocytochemistryLive Cell ImagingRNAi

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