Journal
/
免疫与感染
/
Использование флуоресцентных белков для мониторинга Glycosome Динамика в африканском трипаносомы
JoVE 杂志
免疫与感染
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 免疫与感染
Using Fluorescent Proteins to Monitor Glycosome Dynamics in the African Trypanosome
Please note that all translations are automatically generated. Click here for the English version.

Использование флуоресцентных белков для мониторинга Glycosome Динамика в африканском трипаносомы

8,869 Views

•

10:04 min

•

August 19, 2014

DOI:

10.3791/51647-v

DOI:
10.3791/51647-v

•

10:04 min
August 19, 2014

•

6 Views
, ,
1Department of Genetics and Biochemistry,Clemson University Eukaryotic Pathogens Innovation Center

Summary

Automatically generated

Glycosome dynamics in African trypanosomes are difficult to study by traditional cell biology techniques such as electron and fluorescence microscopy. As a means of observing dynamic organelle behavior, a fluorescent-organelle reporter system has been used in conjunction with flow cytometry to monitor real-time glycosome dynamics in live parasites.

Explore More Videos

Fluorescent ProteinsGlycosome DynamicsTrypanosoma BruceiHuman African TrypanosomiasisNaganaPeroxisomesGlycolysisFluorescence MicroscopyPTS2-eYFPFlow CytometryOrganelle Composition

Read Article

Cite this Article

Bauer, S., Conlon, M., Morris, M. Using Fluorescent Proteins to Monitor Glycosome Dynamics in the African Trypanosome. J. Vis. Exp. (90), e51647, doi:10.3791/51647 (2014).

Download .ris file

Copy

Share Video

.

Copy