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上皮钠通道（ENaC）的蛋白水解激活通过结合电流测量与检测裂解片段示范
JoVE 杂志
生物学
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JoVE 杂志 生物学
Demonstration of Proteolytic Activation of the Epithelial Sodium Channel (ENaC) by Combining Current Measurements with Detection of Cleavage Fragments
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上皮钠通道（ENaC）的蛋白水解激活通过结合电流测量与检测裂解片段示范

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08:56 min

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July 05, 2014

DOI:

10.3791/51582-v

DOI:
10.3791/51582-v

•

08:56 min
July 05, 2014

•

3 Views
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1Institut für Zelluläre und Molekulare Physiologie,Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU)

Summary

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上皮钠通道（ENaC）的异源表达在非洲爪蟾的蛋白水解活化蟾卵母细胞可通过将当前测量与生物素化的方法，调查的离子通道的酶切产物在细胞表面的外观来证明。功能重要的切割位点可通过使用位点定向诱变来识别。

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Proteolytic ActivationEpithelial Sodium Channel (ENaC)Two-electrode Voltage-clampAmiloride-sensitive CurrentProteolytic CleavageBiotinylationGamma-ENaC Fragment

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Cite this Article

Krappitz, M., Korbmacher, C., Haerteis, S. Demonstration of Proteolytic Activation of the Epithelial Sodium Channel (ENaC) by Combining Current Measurements with Detection of Cleavage Fragments. J. Vis. Exp. (89), e51582, doi:10.3791/51582 (2014).

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