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Two-Photon in vivo Imaging of Dendritic Spines in the Mouse Cortex Using a Thinned-skull Preparation
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神经科学
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JoVE 杂志 神经科学
Two-Photon in vivo Imaging of Dendritic Spines in the Mouse Cortex Using a Thinned-skull Preparation

Two-Photon in vivo Imaging of Dendritic Spines in the Mouse Cortex Using a Thinned-skull Preparation

DOI:
10.3791/51520-v

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09:53 min

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May 12, 2014

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,
1Department of Molecular Cell and Developmental Biology,University of California, Santa Cruz

Chapters

  • 00:05Title
  • 00:56Surgery
  • 01:46Thinned-skull Preparation
  • 03:27Immobilization
  • 04:45Imaging
  • 06:33Recovery
  • 07:14Re-imaging
  • 08:22Results: Transcranial Two-Photon Imaging Showing Dynamics of Dendritic Spines
  • 09:31Conclusion

Summary

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自动翻译

Time-lapse imaging in the living animal provides valuable information on structural reorganization in the intact brain. Here, we introduce a thinned-skull preparation that allows transcranial imaging of fluorescently labeled synaptic structures in the living mouse cortex by two-photon microscopy.

Tags

Keywords: Two-photon ImagingIn Vivo ImagingDendritic SpinesMouse CortexThinned-skull PreparationNeuronal PlasticitySynaptic StructureFluorescent LabelingTranscranial ImagingTwo-photon Laser Scanning Microscopy

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