Journal
/
免疫与感染
/
A Method for Generating Pulmonary Neutrophilia Using Aerosolized Lipopolysaccharide
JoVE 杂志
免疫与感染
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 免疫与感染
A Method for Generating Pulmonary Neutrophilia Using Aerosolized Lipopolysaccharide

A Method for Generating Pulmonary Neutrophilia Using Aerosolized Lipopolysaccharide

DOI:
10.3791/51470-v

•

08:33 min

•

December 15, 2014

•

, , , ,
1Department of Medicine, Solna and CMM, Respiratory Medicine Unit,Karolinska Institutet, 2Safety Science, Global Regulator Affairs & Patient Safety,AstraZeneca Global Medicines Development

Chapters

  • 00:05Title
  • 01:13Generating an LPS Aerosol
  • 03:14Bronchoalveolar Lavage (BAG)
  • 05:12Formalin Fixation of Lung Tissue for Histological Assessment
  • 06:26Results: Representative Pulmonary Neutrophilia Data
  • 08:04Conclusion

Summary

自动翻译

自动翻译

We describe a method for inducing neutrophilic pulmonary inflammation by challenge to aerosolized lipopolysaccharide by nebulization, to model acute lung injury. In addition, basic surgical techniques for lung isolation, tracheal intubation and bronchoalveolar lavage are also described.

Tags

Pulmonary NeutrophiliaAerosolized LipopolysaccharideAcute Lung InjuryExperimental ModelPseudomonas AeruginosaBronchoalveolar LavageSterile Pulmonary Inflammation

Article

Embed

添加到播放列表

Usage Statistics

Related Videos

A Method for Labeling Vasculature in Embryonic Mice

Diagnosing Pulmonary Tuberculosis with the Xpert MTB/RIF Test

Peptide:MHC Tetramer-based Enrichment of Epitope-specific T cells

Cecal Ligation and Puncture-induced Sepsis as a Model To Study Autophagy in Mice

Selective Harvesting of Marginating-pulmonary Leukocytes

Two-photon Intravital Imaging of Leukocytes During the Immune Response in Lipopolysaccharide-treated Mouse Liver

Generating Recombinant Avian Herpesvirus Vectors with CRISPR/Cas9 Gene Editing

Co-immunoprecipitation Assay for Studying Functional Interactions Between Receptors and Enzymes

Novel Protocol for Generating Physiologic Immunogenic Dendritic Cells

Visualization of Bacterial Resistance using Fluorescent Antibiotic Probes

Read Article