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Juxtacellular Monitoring en lokalisatie van Single neuronen in Sub-corticale hersenstructuren van Alert, Head ingetogen Ratten
JoVE 杂志
神经科学
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JoVE 杂志 神经科学
Juxtacellular Monitoring and Localization of Single Neurons within Sub-cortical Brain Structures of Alert, Head-restrained Rats
DOI:

08:41 min

April 27, 2015

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Chapters

  • 00:05Title
  • 01:18Implanting the Recording Chamber and Head-restraint Mechanism
  • 05:01Juxtacellular Monitoring of Neuronal Units
  • 07:34Results: Neuronal Units in Ventral Posterior Medial Thalamus Encode the Phase of Vibrissa Movement During Self-generated Whisking
  • 08:13Conclusion

Summary

自动翻译

This protocol describes the design and surgical implantation of a head-restraining mechanism to monitor neuronal activity in sub-cortical brain structures in alert rats. It delineates procedures to isolate single neurons in the juxtacellular configuration and to efficiently identify their anatomical locations.

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