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Chapters
- 00:05Title
- 01:17Dissection and Embedding of the Embryonic Brains
- 02:24Embedded Brain Sectioning and Staining
- 03:59Live Imaging of the Slices
- 04:55Post-acquisition Analysis of Mitosis
- 07:34Results: Live Imaging of Mitosis in the Developing Mouse Embryonic Cortex
- 08:43Conclusion
Neural progenitor mitosis is a critical parameter of neurogenesis. Much of our understanding of neural progenitor mitosis is based on analysis of fixed tissue. Live imaging in embryonic brain slices is a versatile technique to assess mitosis with high temporal and spatial resolution in a controlled environment.
