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激活和NLRP3炎性活性的人单核细胞来源的树突状细胞用IL-1β测量
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JoVE 杂志 免疫与感染
Activation and Measurement of NLRP3 Inflammasome Activity Using IL-1β in Human Monocyte-derived Dendritic Cells
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激活和NLRP3炎性活性的人单核细胞来源的树突状细胞用IL-1β测量

20,628 Views

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09:04 min

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May 22, 2014

DOI:

10.3791/51284-v

DOI:
10.3791/51284-v

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09:04 min
May 22, 2014

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4 Views
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1Department of Pathology,New York University School of Medicine, 2Division of Infectious Diseases, Department of Medicine,Mount Sinai Medical Center, 3Division of Hematology and Oncology, Hess Center for Science and Medicine,Mount Sinai Medical Center

Summary

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树突状细胞（DC）分泌IL-1β响应于TLR8识别合成嘌呤，R848，接着NLRP3炎性体激活与尼日利亚菌素，因此，IL-1β可以用来测量NLRP3炎性活性。细胞内细胞因子染色法，免疫印迹和ELISA法用于准确地测量通过IL-1β表达NLRP3炎性引发和活化。

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Cite this Article

Fernandez, M. V., Miller, E. A., Bhardwaj, N. Activation and Measurement of NLRP3 Inflammasome Activity Using IL-1β in Human Monocyte-derived Dendritic Cells. J. Vis. Exp. (87), e51284, doi:10.3791/51284 (2014).

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