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Whole Mount Immunolabeling of Olfactory Receptor Neurons in the Drosophila Antenna
JoVE 杂志
神经科学
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JoVE 杂志 神经科学
Whole Mount Immunolabeling of Olfactory Receptor Neurons in the Drosophila Antenna

Whole Mount Immunolabeling of Olfactory Receptor Neurons in the Drosophila Antenna

DOI:
10.3791/51245-v

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05:21 min

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May 04, 2014

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, , ,
1Laboratory for Genetic Code, Graduate School of Life and Medical Sciences,Doshisha University, 2Laboratory for Circuit Mechanisms of Sensory Perception,RIKEN Brain Science Institute, 3Disease Mechanism Research Core,RIKEN Brain Science Institute

Chapters

  • 00:00Title
  • 01:20Antenna Dissection
  • 02:50Blocking, Staining, and Mounting of Antenna
  • 04:50Fluorescent Staining
  • 05:00Conclusion

Summary

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Herein we describe the process of whole mount immunostaining of Drosophila antennae, which enables us to better understand the molecular mechanisms involved in the diversification of olfactory receptor neurons (ORN)s.

Tags

Keywords: Whole Mount ImmunolabelingOlfactory Receptor NeuronsDrosophila AntennaOdorant MoleculesReceptor RecognitionOlfactory InformationCellular TransductionTranscriptional FactorsOlfactory Receptor Neuron OrganizationELAV AntibodyOr49a-mCD8::GFPNba NeuronAnti-GFP Antibody

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