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Following in Real Time the Impact of Pneumococcal Virulence Factors in an Acute Mouse Pneumonia Model Using Bioluminescent Bacteria
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JoVE 杂志 免疫与感染
Following in Real Time the Impact of Pneumococcal Virulence Factors in an Acute Mouse Pneumonia Model Using Bioluminescent Bacteria

Following in Real Time the Impact of Pneumococcal Virulence Factors in an Acute Mouse Pneumonia Model Using Bioluminescent Bacteria

DOI:
10.3791/51174-v

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11:32 min

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February 23, 2014

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, , , , , ,
1Department Genetics of Microorganisms, Interfaculty Institute for Genetics and Functional Genomics,University of Greifswald

Chapters

  • 00:05Title
  • 02:23Preparation of the Infectious Stock of Bioluminescent Pneumococci
  • 03:58Intranasal and Intraperitoneal Infection of Mice
  • 05:38Imaging Pneumococcal Dissemination into the Lung and Blood
  • 07:30Results: Pneumococcal Dissemination in Mice after Intranasal Infection
  • 10:14Conclusion

Summary

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Streptococcus pneumoniae is the leading pathogen causing severe community-acquired pneumonia and responsible for over 2 million deaths worldwide. The impact of bacterial factors implicated in fitness or virulence can be monitored in real-time in an acute mouse pneumonia or bacteremia model using bioluminescent bacteria.

Tags

PneumoniaPneumococcusStreptococcus PneumoniaeVirulence FactorsBioluminescenceMouse ModelAcute InfectionReal-time MonitoringDisseminationBacteremia

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