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Detection of the Genome and Transcripts of a Persistent DNA Virus in Neuronal Tissues by Fluorescent In situ Hybridization Combined with Immunostaining
JoVE 杂志
神经科学
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JoVE 杂志 神经科学
Detection of the Genome and Transcripts of a Persistent DNA Virus in Neuronal Tissues by Fluorescent In situ Hybridization Combined with Immunostaining

Detection of the Genome and Transcripts of a Persistent DNA Virus in Neuronal Tissues by Fluorescent In situ Hybridization Combined with Immunostaining

DOI:
10.3791/51091-v

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13:22 min

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January 23, 2014

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, , ,
1Virus and Centromere Team, Centre de Génétique et Physiologie Moléculaire et Cellulaire,CNRS UMR 5534, 2Université de Lyon 1, 3Laboratoire d’excellence,LabEX DEVweCAN, 4Institut de Virologie Moléculaire et Structurale,CNRS UPR 3296, 5Centre de Recherche en Cancérologie de Lyon, INSERM U1052,CNRS UMR 5286

Chapters

  • 00:05Title
  • 02:27Animal Infection
  • 03:20Trigeminal Ganglia Harvesting and Sectioning
  • 05:02DNA-FISH
  • 10:08Protocol Development and Applications
  • 12:32Conclusion

Summary

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We established a fluorescent in situ hybridization protocol for the detection of a persistent DNA virus genome within tissue sections of animal models. This protocol enables studying infection process by codetection of the viral genome, its RNA products, and viral or cellular proteins within single cells.

Tags

Keywords: DNA-FISHRNA-FISHImmunofluorescenceHSV-1LatencyGene Expression RegulationSingle Cell Analysis

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