We provide a technique to preserve intact tree phloem and prepare it for observation. We create an apparatus called a phloem sandwich that allows for the introduction and observation of arthropods, microbes, and other organisms that inhabit phloem tissues.
Aflitto, N. C., Hofstetter, R. W., McGuire, R., Dunn, D. D., Potter, K. A. Technique for Studying Arthropod and Microbial Communities within Tree Tissues. J. Vis. Exp. (93), e50793, doi:10.3791/50793 (2014).