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Chapters
- 00:05Title
- 01:05Preparations
- 02:30Sexing and Dissecting E18 Embryos
- 06:32Neuronal Dissociation
- 07:26Plating and Culture Maintenance
- 08:45Confirming the Sex of the Primary Culture Neurons
- 10:22Conclusion
Primary disassociated embryonic hippocampal neuronal cultures are useful for investigating the signaling mechanisms involved in neuron death. Sexing the embryos before the isolation and dissociation of the hippocampus allows the preparation of separate male and female cultures, which enables the researcher to identify and investigate sex-specific cell signaling.
