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Deriving the Time Course of Glutamate Clearance with a Deconvolution Analysis of Astrocytic Transporter Currents
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神经科学
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JoVE 杂志 神经科学
Deriving the Time Course of Glutamate Clearance with a Deconvolution Analysis of Astrocytic Transporter Currents
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Deriving the Time Course of Glutamate Clearance with a Deconvolution Analysis of Astrocytic Transporter Currents

DOI:
10.3791/50708-v

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09:42 min

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August 07, 2013

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1Synaptic Physiology Section, National Institute of Neurological Disorders and Stroke,National Institutes of Health

Chapters

  • 00:05Title
  • 02:15Slice Preparation
  • 03:54Astrocyte Identification and Recordings
  • 04:46Isolation of the Facilitated Portion of Synaptically-activated Transporter Currents
  • 07:15Isolation of Flash-activated Transporter Currents
  • 07:58Results: Deconvolution Analysis
  • 08:58Conclusion

Summary

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自动翻译

We describe an analytical method to estimate the lifetime of glutamate at astrocytic membranes from electrophysiological recordings of glutamate transporter currents in astrocytes.

Tags

Glutamate ClearanceAstrocytic Transporter CurrentsGlutamate TransportersAstrocytesWhole-cell Patch-clamp RecordingsGlutamate Concentration ProfileTransporter KineticsElectrotonic PropertiesGlutamate UncagingSynaptic Glutamate ReleaseCentral Nervous SystemHealthDisease

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