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Automated High-throughput Behavioral Analyses in Zebrafish Larvae
JoVE 杂志
神经科学
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JoVE 杂志 神经科学
Automated High-throughput Behavioral Analyses in Zebrafish Larvae
DOI:

09:28 min

July 04, 2013

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Chapters

  • 00:05Title
  • 01:48Collection of Zebrafish Embryos and Raising Larvae
  • 03:08Preparing Molds for Behavioral Analyses
  • 04:02Preparation of Imaging Cabinet
  • 04:39Image Capture
  • 06:08Image Analysis
  • 07:31Results: Example of Behaviors that Can Be Quantified in the High-throughput Assay
  • 08:57Conclusion

Summary

自动翻译

Our laboratory developed a novel high-throughput automated imaging system that is useful for the detection of several different behaviors in 7-day-old zebrafish larvae. The system can be used for detecting subtle changes in behavior after the larvae have been exposed to environmental toxicants or pharmaceuticals.

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