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Chapters
- 00:05Title
- 02:40Amplification of the Template
- 04:01Vacuum Workstation Protocol
- 04:05Immobilizing the Biotinylated PCR Products
- 05:06Denaturation of DNA and Addition of Sequencing Primer
- 07:30Annealing Sequencing Primer to DNA Strands
- 07:55Pyromark Q24 Operation for Pyrosequencing
- 07:58Preparing for a Run
- 08:58Starting a Run
- 09:46Completing a Run
- 10:03Results: Pyrosequencing Identifies Bacteria and Detects Antibiotic Drug Resistance
- 11:35Conclusion
Pyrosequencing er en alsidig teknik, der letter mikrobiel genomsekvensering, der kan bruges til at identificere bakteriearter, diskriminere bakteriestammer, og påvise genetiske mutationer, som giver resistens over for antimikrobielle midler. I denne video, vil proceduren for mikrobiel amplicon generation amplikon pyrosekventering, og DNA sekvensanalyse påvises.
Tags
PyrosequencingMicrobial IdentificationMicrobial CharacterizationBacterial Species IdentificationBacterial Strain DiscriminationAntimicrobial Resistance DetectionHigh-throughput ScreeningMicrobial Genome SequencingSingle Base SequencingChemiluminescent DetectionReal-time SequencingSNP TypingMutation Detection
