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Visualisering og analyse av mRNA molekyler med fluorescens<em> In Situ</em> Hybridisering i<em> Saccharomyces cerevisiae</em
JoVE 杂志
生物学
This content is Free Access.
JoVE 杂志 生物学
Visualization and Analysis of mRNA Molecules Using Fluorescence In Situ Hybridization in Saccharomyces cerevisiae

Visualisering og analyse av mRNA molekyler med fluorescens<em> In Situ</em> Hybridisering i<em> Saccharomyces cerevisiae</em

DOI:
10.3791/50382-v

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07:00 min

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June 14, 2013

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, , , , , ,
1The Lewis-Sigler Institute for Integrative Genomics,Princeton University, 2Graduate Program in Quantitative and Computational Biology,Princeton University, 3Department of Molecular Biology,Princeton University

Chapters

  • 00:05Title
  • 01:11Coverslip Preparation
  • 01:51Fixation
  • 03:08Hybridization
  • 04:53Imaging of Cells with Olympus IX-81 Inverted Microscope
  • 05:23Results: Quantitative mRNA Analysis by FISH
  • 06:31Conclusion

Summary

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自动翻译

Denne protokollen beskriver en eksperimentell prosedyre for å utføre Fluorescens<em> In situ</em> Hybridisering (FISH) for telling mRNA i enkeltceller på enkelt-molekyl oppløsning.

Tags

Fluorescence In Situ HybridizationFISHMRNASaccharomyces CerevisiaeSingle-cell AnalysisSingle-moleculeSpot DetectionGene ExpressionVisualizationQuantification

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