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Chapters
- 00:05Title
- 01:44Regulatory T cell isolation from HIV-1 Positive Blood
- 03:40Cell Culture
- 04:27Phenotyping
- 05:14Suppression Assay
- 07:18Results: Representative Treg Cells Expanded from HIV-1-infected Individuals
- 08:33Conclusion
CD4+ Regulatory T cells are potent immune-modulators and serve important functions in immune homeostasis. The paucity of these cells in peripheral blood makes functional studies challenging, specifically in the context of HIV-1-infection. We here describe a method to isolate and expand functional CD4+ Tregs from peripheral blood from HIV-1-infected individuals.
